Journal: Molecular immunology

Article Title: S100A11 regulates nasal epithelial cell remodeling and inflammation in CRSwNPs via the RAGE-mediated AMPK-STAT3 pathway.

doi: 10.1016/j.molimm.2021.09.014

Figure Lengend Snippet: Fig. 5. S100A11 depends on RAGE-mediated can activate AMPK and inhibit STAT3 signaling pathway. A. The phosphorylation levels of AMPK and STAT3 were detected by Western blot after S100A11 was overexpressed. B. The histogram shows the phosphorylation ratio of AMPK and STAT3 (pAMPK/ AMPK and pSTAT3/ STAT3). C. Western blot showed the expression of cleaved-PARP, CCND1, CCNE, Bcl-2, AMPK and STAT3 phosphorylated proteins after treatment of cells with different concentrations of rh-S100A11 protein (0~10000ug/mL) for 24 h. D. Cell immunofluorescence staining detected the expression of STAT3 phosphorylated protein after treatment with 1000ug/mL rh-S100A11 protein for 24 h (bar = 20um). E. The relative mRNA levels of Fas, Stk35, Tsc2 and Socs3 after cells were treated with 1000ug/mL rh-S100A11 protein or transfected for 24 h. F. RAGE protein inhibitors affect the phosphorylation levels of AMPK and STAT3 caused by rhS100A11. The bar graphs show quantification of the results, with each value represents the mean ± SD of three independent experiments. Statistical significance is shown using the Student’s t-test analysis, *P < 0.05; **P < 0.01; ***P < 0.001.

Article Snippet: Rabbit polyclonal anti− CCND1 antibody (Cat. No. 60,186-1-Ig, 1:2000 diluted), anti-RAGE antibody (Cat. No. 66,833-1-Ig, 1:2000 diluted) and anti− CCNE antibody (Cat. No. 11554− 1-AP, 1:2000 diluted for WB) were purchased from Proteintech.

Techniques: Phospho-proteomics, Western Blot, Expressing, Immunofluorescence, Staining, Transfection

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: circFOXM1 promotes proliferation of non-small cell lung carcinoma cells by acting as a ceRNA to upregulate FAM83D

doi: 10.1186/s13046-020-01555-5

Figure Lengend Snippet: Identification and characteristic of circFOXM1 in NSCLC. a circFOXM1 was one of the most upregulated circRNAs based on the analysis of the5 paired samples of NSCLC tissues in GSE126533. T, tumor tissue; N, nontumor tissue. b Relative fold change (T/N) of circFOXM1 in GSE126533. c Expression of circFOXM1 was determined in 48 paired samples of NSCLC tissues. β-actin was used as control. d Histogram and pie chart of the proportions of NSCLC samples in which circFOXM1 expression was upregulated (42/48, 87.50%, red), downregulated (1/48, 2.08%, blue), or no change (5/48, 10.42%, brown). Log2 (T/N) expression value > 1 as higher expression, which < − 1 as lower expression, and between − 1 and 1 as no significant change. e PCR analysis for circFOXM1 in cDNA and genomic DNA (gDNA). f A schematic view of circFOXM1 genomic loci. circFOXM1 is produced at the FOXM1 gene (NM_202002) locus containing exons 4–5. The back-splice junction of circFOXM1 was identified by Sanger sequencing. g RT-PCR analysis for circFOXM1 and FOXM1 mRNA after treatment with RNase R in NSCLC cells. h qRT-PCR analysis for circFOXM1 after treatment with RNase R in NSCLC cells. ** P < 0.01

Article Snippet: The membrane was incubated with rabbit anti-FAM83D antibody (ab236882, Abcam) or anti-CCND1 antibody (26939–1-AP, proteintech) or anti-CCNE1(11554–1-AP, proteintech) or anti-FOXM1 antibody (13147–1-AP, proteintech) or anti-β-actin antibody (20536–1-AP, proteintech) at 4 °C for 12 h, followed by an incubation with secondary antibody (proteintech) for 1 h. Bands were detected by a Bio-Rad ChemiDoc XRS system.

Techniques: Expressing, Control, Produced, Sequencing, Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR